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Transcriptomic analysis of thermally stressed Symbiodinium reveals differential expression of stress and metabolism genes

Chapter 2 of the author’s PhD thesis is published in the journal Frontiers in Plant Science. This study describes the transcriptome response of Symbiodinium sp. (clade F) cultures exposed to thermal stress (four, nineteen and twenty-eight days) at future temperature conditions and is linked with physiological changes observed. Within the differentially expressed genes, transcripts with significance to the stress response of Symbiodinium were detected. The results indicate a shift in metabolism, from carbon fixation to fatty acid catabolism, supported by upregulation of β-oxidation, glyoxylate cycle and gluconeogenic enzymes, this to our knowledge, has not previously been reported in Symbiodinium. The implications of altered metabolic processes from exposure to thermal stress found in this study, on coral – Symbiodinium associations has not been explored. This study provides an important reference for understanding the mechanisms of coral bleaching at future temperature conditions. The Illumina sequenced read data reported in this article have been deposited into the National Center for Biotechnology Information (NCBI) Sequence Read Archive under the accession number SRA467551, which is associated with BioProject number PRJNA342240. The associated analysed data included as appendices of this thesis may be found in the supplementary information of the publication or attached as MS Excel sheets.
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    Data record related to this publication Transcriptomic analysis of thermally stressed Symbiodinium reveals differential expression of stress and metabolism genes
    Data Publication title Transcriptomic analysis of thermally stressed Symbiodinium reveals differential expression of stress and metabolism genes
  • Description

    Chapter 2 of the author’s PhD thesis is published in the journal Frontiers in Plant Science. This study describes the transcriptome response of Symbiodinium sp. (clade F) cultures exposed to thermal stress (four, nineteen and twenty-eight days) at future temperature conditions and is linked with physiological changes observed. Within the differentially expressed genes, transcripts with significance to the stress response of Symbiodinium were detected. The results indicate a shift in metabolism, from carbon fixation to fatty acid catabolism, supported by upregulation of β-oxidation, glyoxylate cycle and gluconeogenic enzymes, this to our knowledge, has not previously been reported in Symbiodinium. The implications of altered metabolic processes from exposure to thermal stress found in this study, on coral – Symbiodinium associations has not been explored. This study provides an important reference for understanding the mechanisms of coral bleaching at future temperature conditions. The Illumina sequenced read data reported in this article have been deposited into the National Center for Biotechnology Information (NCBI) Sequence Read Archive under the accession number SRA467551, which is associated with BioProject number PRJNA342240. The associated analysed data included as appendices of this thesis may be found in the supplementary information of the publication or attached as MS Excel sheets.

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    • Descriptor

      This dataset consists of 5 spreadsheets in MS Excel (.xlsx) and Open Document formats (.ods)

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    • Data type dataset
  • Keywords
    • Symbiodinium
    • gene expression
    • RNA-Seq
    • transcriptome
    • thermal stress
    • ARC Centre of Excellence for Coral Reef Studies
  • Funding source
  • Research grant(s)/Scheme name(s)
    • CE140100020 - Australian Research Council Centre of Excellence for Integrated Coral Reef Sciences
    • DP130101421 - ARC Discovery Project: Advancing knowledge of microbial symbioses underpinning coral health and reef resilience and predicting their responses to climate change
    • DP160100271 - ARC Discovery Project: Photosynthetic traits as "key performance indicators" of coral health
    • Research themes
    Tropical Ecosystems, Conservation and Climate Change
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      Name Gierz, Sarah, Forêt, Sylvain, and Leggat, William (2017) Transcriptomic analysis of thermally stressed Symbiodinium reveals differential expression of stress and metabolism genes. Frontiers in Plant Science, 8. pp. 1-20.
    • URL http://dx.doi.org/10.3389/fpls.2017.00271
    • Notes Open Access
      • Name Gierz, Sarah L., Gordon, Benjamin R., and Leggat, William (2016) Integral light-harvesting complex expression in Symbiodinium within the coral Acropora aspera under thermal stress. Scientific Reports, 6:25081.
    • URL http://dx.doi.org/10.1038/srep25081
    • Notes Open Access; no direct relationship to this dataset
      • Name Gierz, Sarah Louise (2017) Thermal acclimation and light-harvesting complex expression in Symbiodinium. PhD thesis, James Cook University.
    • URL https://researchonline.jcu.edu.au/51805/
    • Notes Open Access via ResearchOnline@JCU
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    The data will be licensed under CC BY: Attribution 3.0 AU
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  • Data owners
      Sarah Gierz
- Citation
    Citation Gierz, Sarah (2017): Transcriptomic analysis of thermally stressed Symbiodinium reveals differential expression of stress and metabolism genes. James Cook University. https://doi.org/10.4225/28/598a96f71288a