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Data from: Destabilization of mutualistic interactions shapes the early heat stress response of the coral holobiont

Background: This study explores the stability of the symbiotic relationship between coral and their dinoflagellate algae (Symbiodiniaceae) under increasing heat stress. In a 2-month manipulative experiment, the thermotolerant coral Porites lutea was exposed to increasing temperatures, from 28˚C to 32˚C (0 – 8 Degree Heating Weeks, DHW), and the stability of the host-symbiont relationship was assessed through coral and Symbiodiniaceae transcriptomics, microbial 16S rRNA gene sequencing and physiological measurements. 

The data record contains:

  • link to the NCBI BioProject containing raw sequence data generated from 16S rRNA gene sequencing (PRJNA1050834)
  • link to the NCBI BioProject containing raw sequence data generated from mRNA sequencing (PRJNA1050834)
  • one csv file with the coral Health score used in the analyses
  • one csv file with the photosynthesis and respiration data
  • one csv file with the Symbiodiniaceae photochemical effective efficiency data
  • link to the GitHub repository with the R scripts generated for the analyses

Software/equipment used to create/collect the data: 

  • 16S rRNA amplification (515F-806rB primers) of gDNA from coral (Porites lutea) and seawater samples; Illumina MiSeq sequencing (2x250 bp).
  • RNA sequencing from coral samples; NovaSeq 6000 S2 (2 x 100 bp)
  • health score assessment over time using the CoralWatch Coral Health Chart (Siebeck et al 2006)
  • measures of net photosynthesis and respiration rates over time by assessing oxygen concentration using an Optical probe after placing corals in 600 mL plastic chambers for 50-min incubation under light conditions (130 µmol photons m−2 s−1) and 75-min incubation in dark conditions, respectively.
  • measures of photochemical effective efficiency over time using a mini-PAM fluorometer (Walz, Germany; settings: MI = 4; SI = 8; SW = 0.8; G=2; D=2) 

Software/equipment used to manipulate/analyse the data: 

  • amplicon paired-end reads were analysed in QIIME 2 (v2020.8), and output data were imported and analysed in R (v 4.0.3).
  • reference-based transcirptomics was applied using the RNA-seq aligner STAR, and output data were imported and analysed in R (v 4.0.3).
  • physiological measures were analysed in R (v 4.0.3).
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    Data Record Details
    Data record related to this publication Coral holobiont heat stress responses
    Data Publication title Data from: Destabilization of mutualistic interactions shapes the early heat stress response of the coral holobiont
  • Description

    Background: This study explores the stability of the symbiotic relationship between coral and their dinoflagellate algae (Symbiodiniaceae) under increasing heat stress. In a 2-month manipulative experiment, the thermotolerant coral Porites lutea was exposed to increasing temperatures, from 28˚C to 32˚C (0 – 8 Degree Heating Weeks, DHW), and the stability of the host-symbiont relationship was assessed through coral and Symbiodiniaceae transcriptomics, microbial 16S rRNA gene sequencing and physiological measurements. 

    The data record contains:

    • link to the NCBI BioProject containing raw sequence data generated from 16S rRNA gene sequencing (PRJNA1050834)
    • link to the NCBI BioProject containing raw sequence data generated from mRNA sequencing (PRJNA1050834)
    • one csv file with the coral Health score used in the analyses
    • one csv file with the photosynthesis and respiration data
    • one csv file with the Symbiodiniaceae photochemical effective efficiency data
    • link to the GitHub repository with the R scripts generated for the analyses

    Software/equipment used to create/collect the data: 

    • 16S rRNA amplification (515F-806rB primers) of gDNA from coral (Porites lutea) and seawater samples; Illumina MiSeq sequencing (2x250 bp).
    • RNA sequencing from coral samples; NovaSeq 6000 S2 (2 x 100 bp)
    • health score assessment over time using the CoralWatch Coral Health Chart (Siebeck et al 2006)
    • measures of net photosynthesis and respiration rates over time by assessing oxygen concentration using an Optical probe after placing corals in 600 mL plastic chambers for 50-min incubation under light conditions (130 µmol photons m−2 s−1) and 75-min incubation in dark conditions, respectively.
    • measures of photochemical effective efficiency over time using a mini-PAM fluorometer (Walz, Germany; settings: MI = 4; SI = 8; SW = 0.8; G=2; D=2) 

    Software/equipment used to manipulate/analyse the data: 

    • amplicon paired-end reads were analysed in QIIME 2 (v2020.8), and output data were imported and analysed in R (v 4.0.3).
    • reference-based transcirptomics was applied using the RNA-seq aligner STAR, and output data were imported and analysed in R (v 4.0.3).
    • physiological measures were analysed in R (v 4.0.3).
    •
  • Other Descriptors
    • Descriptor
      • Descriptor type
    • Data type dataset
  • Keywords
    • Coral
    • Heat stress
    • Stress intensity
    • Symbiodiniaceae
    • Microbiome
    • Gene expression profiling
    • Transcriptomics
    • Physiology
    • Holobiont
    • Symbiosis
  • Funding source
    • Australian Institue of Marine Science (Evolution21 project)
  • Research grant(s)/Scheme name(s)
    • - Hunter Research Grant
    • - JCU CSE Competitive Research Training Grant
    • Research themes
    Tropical Ecosystems, Conservation and Climate Change
    FoR Codes (*)
    • 410102 - Ecological impacts of climate change and ecological adaptation
    • 310703 - Microbial ecology
    • 310505 - Gene expression (incl. microarray and other genome-wide approaches)
    SEO Codes
    • 190199 - Adaptation to climate change not elsewhere classified
- Specify coverage
    Specify spatial or temporal setting of the data
    Temporal (time) coverage
  • Start Date 2019/09/27
  • End Date 2020/02/26
    • Time Period
    Spatial (location) coverage
  • Locations
    • Great Barrier Reef, Australia
- Data
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- License
- Citation
    Citation Marangon, Emma; Li, Joan; Webster, Nicole; Bourne, David; Laffy, Patrick (2024): Data from: Destabilization of mutualistic interactions shapes the early heat stress response of the coral holobiont. James Cook University. https://doi.org/10.25903/c5j3-1p16