METADATA for Downie AT, Phelps CM, Illing B, Whan J, Cowman PF, McCormick MI, Rummer JL (in prep) The physiological mechanisms underpinning metamorphosis in reef fishes (PNAS: target)

Methodology: The study investigated changes in swimming performance, oxygen uptake and muscle mitochondria volume density of larval and juvenile ambon damselfishes.
             The study was conducted on Lizard Island, Australia. Light traps were deploy in the aftertoon (16:00) and collected at 22:00 to get larvae and 06:00 to get juveniles.
             Larvae were collected at night to prevent them from metamorphosing. Larvae (n=10) and juveniles (24, 48 and 72 h post-settled onto the reef; n=10 per stage) were swum in
             in the same swimming respirometer.A swimming respirometer is a glass chamber with an installed propellor that generates flow.
             The larvae swim against this flow, and oxygen probes measure the changes in oxygen uptake rates as the fish swim. 
              The larvae would undergo a stepped velocity test (Ucrit; 1 body length per second every 20 minutes) until fatigue; this fatigue speed is the maximum swimming speed that the larvae can support mainly aerobically. 
             At each velocity increment until fatigue, oxygen uptake rate is measured. The oxygen uptake rate at maximum swimming
             speed is Maximum Metabolic Rate (MMR). Extrapolating the oxygen uptake rates at each swimming speed to '0' swimming
             speed provides an estimate of Standard Metabolic Rate (SMR), which are basic maintenance costs. The difference between
             MMR and SMR is aerobic scope (AS), which is an estimate for whole animal energy budgets. Pectoral and trunk muscles (left and right sides of the fish) were dissected out of the fish after the trial. 
             The tissues were perserved in glutaraldehyde and images (electron microgrpahs) of mitochondria were taken for each peice of tissue using electron microscopy (n=10 images per peice of tissue). Mitochondria were quantified via
             point counting techniques, invovling placing a grid ontop of the electron micrograph and counting the number of intersecting points of the grid that touches a mitochondria.  

The data consists of 2 files in comma-seperated values (.csv) format and a metadata file (.txt) that includes all of the variables, labels and units.
The data files are: 

Files:
File Description
DOWNIE_Chpt6_metadata
This file
DOWNIE_PhD_Chpt6_SwimmingRespirometry
Swimming respirometry data for ambon damselfsh larva and metamorphosed juveniles
DOWNIE_PhD_Chpt6_MuscleMitochondria
Muscle mitochondria volume density data for ambon damselfish larva and metamorphosed juveniles

.csv file headings 
Column heading descriptions
Stage
Ontogenetic stage investigated: larvae or juveniles (24, 48 or 72 h post-metamorphosed)
Pulse
One of two collection dates
Fish_ID
Unique identification for each individual fish
Fish# 
Fish number (n=10 for each stage)
Mass
Fish mass (g)
Ucrit
Critical swimming speed (cm s-1)
SMR
Mass-specific standard metabolic rate (mg O2 kg-1h-1) 
MMR
Mass-specific maximum metabolic rate (mg O2 kg-1h-1) 
AAS
Mass-specific absolute aerobic scope (mg O2 kg-1h-1) 
FAS
Mass-specific factorial aerobic scope (mg O2 kg-1h-1) 
Muscle type
Red muscle type (trunk or pectoral) used to quantify muscle mitochondria volume density
Vmt
Muscle mitochondrial volume density (% of muscle tissue volume)